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GSH regulates mTORC1 signaling and the ISR during cystine starvation Given that cystine and GSH balance are tightly coupled, we next evaluated whether GSH is a modulator of cystine-induced cell signaling

The magnitude of response in this system is an integrated composite of the rate of Gs-Venus recruitment and activation (that will release the G protein from the activated receptor)

The eyes were rapidly enucleated, fixed for 2 h in the same solution, and then transferred into a sucrose buffer (30% sucrose in a 0.1 M phosphate buffer) for cryoprotection for 24 h

Gene-expression regulated by epigenetic modifications, such as alter DNA accessibility and chromatin structure, histone modification, and DNA methylation 1,2